52009-10伯优®细胞核分离试剂盒上海伯豪生物技术有限公司服务科技创新，护航人类健康！

产品简介

本产品专为从动物组织中分离高纯度的单细胞核而设计。组织通过匀浆、裂解细胞膜等步骤释放完整细胞核，同时维持核膜稳定性及染色质空间结构，优化的密度梯度离心技术可进一步去除细胞碎片和杂质，从而可满足下游单细胞组学、表观遗传学等前沿研究领域对细胞核的质量要求。

产品应用

制备的细胞核悬液可用于核转录组学（如 snRNA-seq/bulk RNA-seq），表观遗传学（如scATAC- seq/bulk ATAC- seq，CUT&Tag）等研究。

竞品对比测试

伯优®细胞核分离试剂盒单页-03

结果图片

伯优®细胞核分离试剂盒单页-01

应用实例

伯优®细胞核分离试剂盒已成功应用于近20个物种的细胞核分离，涵盖人、小鼠、大鼠等哺乳动物及扇贝、海葵等无脊椎动物。在组织适配性方面，本试剂盒已用于包括脑、肝脏、心脏、肺、肾、脾及各类肿瘤等200余种组织，累计20,000余例样本的细胞核悬液制备。为单细胞核RNA测序提供了稳定可靠的细胞核分离解决方案。

单细胞核RNA测序实测组织类型

伯优®细胞核分离试剂盒单页-02

This product is specially designed to isolate high-purity single nuclei from animal tissues. Intact nuclei are released through tissue homogenization and cell membrane lysis, while the nuclear membrane stability and spatial structure of chromatin are well preserved. The optimized density gradient centrifugation effectively removes cell debris and contaminants, enabling the nuclei to meet strict quality requirements for cutting-edge research including single-cell omics and epigenetics.

Applications

The prepared nuclear suspension is applicable to nuclear transcriptomics (e.g., snRNA-seq, bulk RNA-seq) and epigenetics research (e.g., scATAC-seq, bulk ATAC-seq, CUT&Tag).

Microscopy Images

Application Cases

Boyou® Nucleus Isolation Kit has been successfully used for nucleus extraction from nearly 20 species, including mammals such as humans, mice and rats, as well as invertebrates like scallops and sea anemones. It is compatible with over 200 tissue types including brain, liver, heart, lung, kidney, spleen and various tumor tissues, with more than 20,000 sample preparations completed so far. It provides a stable and reliable solution for single-nucleus RNA sequencing.

Tested Tissue Types for Single-Nucleus RNA Sequencing

应用文献

IF 14.1 Advanced Science 2026

Stage-Specific H3K14 and H3K23 Succinylation Orchestrates Insect Metamorphosis and Oogenesis

产品页

序号: 3012
分类: 试剂
原文: using the Nuclear Isolation Kit (SHBIO)
应用方向: Cut&Tag-seq
产品线: 试剂
服务种类: 细胞核分离试剂盒
使用服务种类: Nuclear Isolation Kit (SHBIO).
作者名: Yu-Pu Jing, Lunjie Li, Libin Yang, Lin-Jie Zhang, Qiang Yan, Ci Zhang, Zanrong Wen, Guangze Yu, Qian Li, Shutang Zhou
文章名称: Stage-Specific H3K14 and H3K23 Succinylation Orchestrates Insect Metamorphosis and Oogenesis
杂志: Advanced Science
IF: 14.1
发表年份: 2026
发表日期: 2026 May 10
Abstract: [Abstract]
类型: 客户发文
备注: 材料和方法提及Shbio
关键词: 繁殖力|GPCR信号传导|组蛋白琥珀酰化|昆虫变态|PKC亚型|翻译后修饰
分类: 农学
研究内容: 机制
采样物种分类: 棉铃虫
通讯作者: Shutang Zhou
单位: 河南农业大学
区域: 华北

IF 8 npj Precision Oncology 2026

Single-nucleus sequencing dissects the malignant change of early lung adenocarcinoma: SMAD3 suppresses antitumor T cell immunity via ICAM1

产品页

序号: 3008
分类: 试剂
原文: The nucleus was isolated using a Nucleus Isolation Kit (SHBIO 52009-10) as instructions.
应用方向: snRNA-seq
产品线: 试剂
服务种类: 细胞核分离试剂盒
使用服务种类: Nucleus Isolation Kit (SHBIO 52009-10)
样本类型: 组织
样本详情: 肺
作者名: Zheng Zhi, Liao Shuangqing, Yang Chengfei, Li Yanqi, Wang Kai, Huang Ziqi, Liu Sijin, Dai Zhuoxin, Liu Xiaobing, Dai Peng, Deng Xufeng, Dai Jigang, Liu Quanxing
文章名称: Single-nucleus sequencing dissects the malignant change of early lung adenocarcinoma: SMAD3 suppresses antitumor T cell immunity via ICAM1
杂志: npj Precision Oncology
IF: 8
发表年份: 2026
发表日期: 2026 May 12
Abstract: [Abstract]
类型: 客户发文
平台: SeekOne
备注: 材料和方法提及Shbio
分类: 医学
研究内容: 机制
病种: 肺腺癌
采样物种分类: 人
通讯作者: Ji-Gang Dai
单位: 陆军医科大学新桥医院
区域: 华南

IF 6.9 Cell Reports 2026

Multi-omics analysis uncovers the molecular basis of “golden-thread” formation in Phoebe zhennan stems

产品页

序号: 3006
分类: 试剂
原文: using a Nucleus Isolation Kit (SHBIO, Shanghai, China) as instructions
应用方向: snRNA-seq
产品线: 试剂
服务种类: 细胞核分离试剂盒
使用服务种类: Nucleus Isolation Kit(SHBIO, Shanghai, China)
样本类型: 组织
样本详情: 茎
作者名: Lingfei Kong, Qin Song, Shaoming Liang, Heng Huang, Hongbin Wei, Shunqin Zhu, Yubo Wang, Haoran Lin, Yijin Chen, Ruiying Su, Li Huang, Hanyu Chen, Yinfei Peng, Zhengjie Zhao, Yiling Li, Deyan Wang, Jiale Zhao, Benwen Chen, Hengxing Zhu, Qianli Dai, Yuansong Guo, Huadong Luo, Mingguo Hou, Bin Zhao, Tao Ma, Keming Luo
文章名称: Multi-omics analysis uncovers the molecular basis of “golden-thread” formation in Phoebe zhennan stems
杂志: Cell Reports
IF: 6.9
发表年份: 2026
发表日期: 2026 May 05
Abstract: [Abstract]
类型: 客户发文
平台: SeekOne
备注: 材料和方法提及Shanghai Biotechnology Corporation
分类: 植物
研究内容: 机制
采样物种分类: 楠木
通讯作者: Tao Ma,Keming Luo
单位: 西南大学
区域: 华南

IF 4.3 Frontiers in Cell and Developmental Biology 2026

Cardiomyocyte-derived BDNF restricts cardiac fibrosis by decreasing the activity of the TGF-β/Smad2/3 pathway and increasing Smad7 expression

产品页

序号: 2989
分类: 试剂
原文: Nuclei were isolated using a Shbio Nucleus Isolation Kit (SHBIO, #52009–10).
应用方向: snRNA-seq
产品线: 试剂
服务种类: 细胞核分离试剂盒
使用服务种类: Shbio Nucleus Isolation Kit (SHBIO, #52009–10)
样本类型: 组织
样本详情: 心脏
作者名: Yu Zhu, Yuanfei Ran, Tingting Fu, Xin Zheng, Yanjun Chen, Chunbao Liang, Yanmei Li, Ruijin Huang, Hui Zhao, Xiudi Pan, Ziqiang Yuan, Qin Pu, Zhaohua Zeng, Shanshan Feng, Xufeng Qi, Luocheng Lv, Lixuan Zhan, Yilin Chen, Dongqing Cai
文章名称: Cardiomyocyte-derived BDNF restricts cardiac fibrosis by decreasing the activity of the TGF-β/Smad2/3 pathway and increasing Smad7 expression
杂志: Frontiers in Cell and Developmental Biology
IF: 4.3
发表年份: 2026
发表日期: 2026 Apri 28
Abstract: [Abstract]
类型: 客户发文
平台: 10X
备注: 材料和方法提及Shanghai Biotechnology Corporation
关键词: BDNF TrkB通路、心脏成纤维细胞和心肌成纤维细胞、心脏纤维化、心肌细胞衍生的BDNF条件性敲除、TGF-β/Smad2/3通路
分类: 医学
研究内容: 机制
病种: 心脏纤维化
采样物种分类: 小鼠
通讯作者: Luocheng Lv, Lixuan Zhan10,Yilin Chen1,2,3,4* and Dongqing Cai1,2,3,4
单位: 暨南大学
区域: 华南

IF 42.5 CELL 2026

B cell deficiency limits exercise capacity by remodeling liver glutamate metabolism

产品页

序号: 2986
分类: 试剂
原文: Nucleus Isolation Kit (SHBIO, 52009-10)
应用方向: snRNA-seq
产品线: 试剂
服务种类: 细胞核分离试剂盒
使用服务种类: Nucleus Isolation Kit (SHBIO, 52009-10)
样本类型: 组织
样本详情: 骨骼肌
作者名: Youxiang Mao, Ziyan Xia, Xu Pan, Wenjun Xia, Peng Jiang
文章名称: B cell deficiency limits exercise capacity by remodeling liver glutamate metabolism
杂志: CELL
IF: 42.5
发表年份: 2026
发表日期: 2026 Apri 17
Abstract: [Abstract]
类型: 客户发文
平台: seek one
备注: 材料和方法提及Shanghai Biotechnology Corporation
分类: 医学
研究内容: 机制
采样物种分类: 小鼠
通讯作者: Peng Jiang
单位: 清华大学
区域: 华北

IF 9.4 Genes & Diseases 2026

Rac1 impairs retinal axon degeneration via Kif2a in a mouse model of chronic ocular hypertension

产品页

序号: 2979
分类: 试剂
原文: mice at G1w were isolated with a Nucleus Isolation Kit (Cat#52009-10, SHBIO, Shanghai, China).
应用方向: CUT&Tag
产品线: 试剂
服务种类: 细胞核分离试剂盒
使用服务种类: Nucleus Isolation Kit (Cat#52009-10, SHBIO
作者名: Hong Zhou, Shumin Zhong, Yunhui Guo, Guoli Zhao, Wenwen Ding, Fang Li, Yongchen Wang, Zhongfeng Wang, Yanying Miao
文章名称: Rac1 impairs retinal axon degeneration via Kif2a in a mouse model of chronic ocular hypertension
杂志: Genes & Diseases
IF: 9.4
发表年份: 2026
发表日期: 2026 Apri 30
Abstract: [Abstract]
类型: 客户发文
备注: 材料和方法提及Shbio
关键词: 轴突变性；青光眼；Kif2a；Rac1；视网膜神经节细胞；沉默信息调节因子1
分类: 医学
研究内容: 机制
病种: 青光眼
采样物种分类: 小鼠
通讯作者: Yanying Miao
单位: 复旦大学
区域: 华东

IF 15.7 Nature Communications 2026

Impact of NPAS2 on mPFC dopamine synthesis and nap behavior

产品页

序号: 2963
分类: 试剂
原文: Nuclei were isolated using a Shbio Cell Nuclear Isolation Kit (Shibio, 52009-10)
应用方向: snRNA-seq
产品线: 试剂
服务种类: 细胞核分离试剂盒
使用服务种类: Shbio Cell Nuclear Isolation Kit (Shibio, 52009-10)
样本类型: 组织
样本详情: 脑
作者名: Guo Lianxia, Cen Haobin, Huang Yuwei, Li Zanjin, Zeng Kengran, Wu Zicong, Weng Jiaxian, Guo Xiaocao, He Di, Liu Xinyu, Yang Zhehan, Xu Haiman, Hao Tingying, Wei Binbin, Diao Xingxing, Wu Baojian
文章名称: Impact of NPAS2 on mPFC dopamine synthesis and nap behavior
杂志: Nature Communications
IF: 15.7
发表年份: 2026
发表日期: 2026 Mar 16
Abstract: [Abstract]
类型: 客户发文
平台: 10X
备注: 材料和方法提及Shanghai Biotechnology Corporation
关键词: 午睡；NPAS2；mPFC；TH+神经元；昼夜节律
分类: 医药
研究内容: 机制
采样物种分类: 小鼠
通讯作者: Binbin Wei3,*, Xingxing Diao4,* and Baojian Wu1
单位: 广州医科大学
区域: 华南

IF 2.4 GENE 2026

Differential early tubular injury in minimal Change disease and Focal segmental Glomerulosclerosis: A Multifaceted analysis

产品页

序号: 2946
分类: 单细胞
原文: Shbio Nuclear Isolation Kit (Shbio, #52009–10, Shanghai, China).
应用方向: snRNA-seq
产品线: 单细胞/试剂
服务种类: 单细胞核转录组测序/细胞核分离试剂盒
样本类型: 组织
样本详情: 肾
作者名: Yiting Zhao, Jianbo Qing, Xiao Wang, Yafeng Li, Junnan Wu
文章名称: Differential early tubular injury in minimal Change disease and Focal segmental Glomerulosclerosis: A Multifaceted analysis
杂志: GENE
IF: 2.4
发表年份: 2026
发表日期: 2026 Feb 08
Abstract: [Abstract]
类型: 客户发文
平台: 10X
备注: 材料和方法提及Shanghai Biotechnology Corporation
关键词: 微小病变局灶节段性肾小球硬化近端小管单核RNA测序多重免疫组织化学SLC7A8
分类: 医学
研究内容: 机制
病种: 微小病变（MCD）和局灶节段性肾小球硬化（FSGS）
采样物种分类: 人
通讯作者: Junnan Wu
单位: 浙江大学
区域: 华东

IF 15.7 Nature Communications 2026

Gut microbiota-derived butyrate primes systemic immunity in honey bees by mediating lipid metabolic reprogramming

产品页

序号: 2945
分类: 试剂
原文: Nuclear extraction kit (Cat#52009-10）
应用方向: CUT&Tag
产品线: 试剂
服务种类: 细胞核分离试剂盒
使用服务种类: Nuclear extraction kit (Cat#52009- 10）
样本类型: 微生物
样本详情: 肠道微生物
作者名: Jiaming Liu1, Yashuai Wu1, Zhenfang Li1, Junbo Tang1, Xin Zhou1, Shiqi Luo1
文章名称: Gut microbiota-derived butyrate primes systemic immunity in honey bees by mediating lipid metabolic reprogramming
杂志: Nature Communications
IF: 15.7
发表年份: 2026
发表日期: 2026 Feb 02
Abstract: [Abstract]
类型: 客户发文
备注: 材料和方法提及Shanghai Biotechnology Corporation
分类: 植保
研究内容: 机制
采样物种分类: 蜜蜂
通讯作者: Shiqi Luo
单位: 中国农业大学植保学院
区域: 华北

IF 25.9 CELL RESEARCH 2026

Mitochondrial double-stranded RNA drives aging-associated cognitive decline

产品页

序号: 2944
分类: 试剂
原文: Nucleus Isolation Kit (SHBIO, 52009-10).
应用方向: snRNA-seq
产品线: 试剂
服务种类: 细胞核分离试剂盒
使用服务种类: Nucleus Isolation Kit (SHBIO, 52009-10).
样本类型: 细胞
样本详情: 细胞
作者名: Zhang Lixiao, Li Xiang, Luo Hongdi, Huo Yujia, Zhou Guangkeng, Wang Pengcheng, Wu Sipeng, Lin Xinyong, Dai Kai, Shi Jiahao, Wang Zebao, Xu Jiaxin, Li Renjian, Chen Siyi, Sun Zhe, Zhao Chunlin, Zhou Zizhuo, Wang Zhenhong, Liang Chensi, Zhu Jun, Chen Xingjun, Luo Jintao, Yu Yong, Zhang Zhirong, Wang Geng
文章名称: Mitochondrial double-stranded RNA drives aging-associated cognitive decline
杂志: CELL RESEARCH
IF: 25.9
发表年份: 2026
发表日期: 2026 Feb 16
Abstract: [Abstract]
类型: 客户发文
平台: SeekOne
备注: 材料和方法提及Shbio
分类: 医学
研究内容: 机制
病种: 衰老
采样物种分类: 小鼠
通讯作者: 王耿
单位: 厦门大学
区域: 华南

IF 10.1 Journal of Neuroinflammation 2025

NEO1 modulates the A1 astrocyte polarization in subarachnoid hemorrhage through the cPLA2-MAVS signaling pathway

产品页

序号: 2915
分类: 试剂
原文: Nucleus Isolation Kit (SHBIO 52009-10)
应用方向: snRNA-seq
产品线: 试剂
服务种类: 细胞核分离试剂盒
使用服务种类: Nucleus Isolation Kit (SHBIO 52009-10)
样本类型: 组织
样本详情: 颈动脉
作者名: Yu Wu, Boyang Wei, Lei Jin, Longxiang Li, Zeyu Yang, Can Li, Jiaming Zhou, Wenzhi Gong, Xin Feng, Shenquan Guo, Wenping Cheng, Ran Li, Shuyin Liang, Shixing Su, Fa Jin, Xin Zhang, Yanchao Liu, Chuanzhi Duan, Xifeng Li, Wenchao Liu
文章名称: NEO1 modulates the A1 astrocyte polarization in subarachnoid hemorrhage through the cPLA2-MAVS signaling pathway
杂志: Journal of Neuroinflammation
IF: 10.1
发表年份: 2025
发表日期: 2025 Dec 04
Abstract: [Abstract]
类型: 客户发文
平台: SeekOne
备注: 材料和方法提及Shanghai Biotechnology Corporation
关键词: 蛛网膜下腔出血、神经炎症、星形胶质细胞极化、NEO1、cPLA2、MAVS
分类: 医学
研究内容: 机制
病种: 神经炎症
采样物种分类: 小鼠
通讯作者: Chuanzhi Duan，Xifeng Li, Wenchao Liu
单位: 南方医科大学珠江医院
区域: 华南

IF 6.9 Scientific Data 2025

Comprehensive snRNA-Seq Datasets of Human and Mouse Podocytopathy Integrated with GWAS of Microalbuminuria

产品页

序号: 2912
分类: 试剂
原文: Shbio Nucleus Isolation Kit (Shbio, 52009-10).
应用方向: snRNA-seq
产品线: 试剂
服务种类: 细胞核分离试剂盒
使用服务种类: Shbio Nucleus Isolation Kit (Shbio, 52009-10).
样本类型: 组织
样本详情: 肾
作者名: Qing Jianbo, Hu Jiaying, Wang Xiao, Wu Junnan
文章名称: Comprehensive snRNA-Seq Datasets of Human and Mouse Podocytopathy Integrated with GWAS of Microalbuminuria
杂志: Scientific Data
IF: 6.9
发表年份: 2025
发表日期: 2025 Dec 16
Abstract: [Abstract]
类型: 客户发文
平台: 10x
备注: 材料和方法提及Shanghai Biotechnology Corporation
关键词: 足细胞；足细胞病；单核RNA测序；微量白蛋白尿；治疗目标
分类: 医学
研究内容: 机制
病种: 足细胞病
采样物种分类: 人/小鼠
通讯作者: Junnan Wu
单位: 浙江大学邵逸夫医院
区域: 华东

IF 7.9 Clinical and Translational Medicine 2025

Proteogenomic characterisation of primary oral cancer unveils extracellular matrix remodelling and immunosuppressive microenvironment linked to lymph node metastasis

产品页

序号: 2894
分类: 试剂
原文: Shbio Nuclei Isolation Kit (SHBIO, #52009-10）
应用方向: snRNA-seq
产品线: 试剂
服务种类: 细胞核分离试剂盒
使用服务种类: Shbio Nuclei Isolation Kit (SHBIO, #52009-10
样本类型: 组织
样本详情: 口腔鳞状细胞癌肿瘤组织
作者名: Yu Liu, Zhenyu Yang, Jingya Jane Pu, Jie Zhong, Ui-Soon Khoo, Yu-Xiong Su, Gao Zhang
文章名称: Proteogenomic characterisation of primary oral cancer unveils extracellular matrix remodelling and immunosuppressive microenvironment linked to lymph node metastasis
杂志: Clinical and Translational Medicine
IF: 7.9
发表年份: 2025
发表日期: 2025 Mar 04
Abstract: [Abstract]
类型: 客户发文
平台: 10X
备注: 材料和方法提及Shanghai Biotechnology Corporation
分类: 医学
研究内容: 机制
病种: 口腔鳞状细胞癌
采样物种分类: 人
通讯作者: Jie Zhong
单位: 香港大学
区域: 华南

IF 4.6 Frontiers in Cell and Developmental Biology 2025

snRNA-seq reveals subcutaneous white adipose tissue remodeling upon return to thermoneutrality after cold stimulation

产品页

序号: 2889
分类: 试剂
原文: Shbio Nuclei Isolation Kit (SHBIO，Cat# 52009–10）
应用方向: snRNA-seq
产品线: 试剂
服务种类: 细胞核分离试剂盒
使用服务种类: Shbio Nuclei Isolation Kit (SHBIO）
样本类型: 组织
样本详情: 白色脂肪组织
作者名: Yusha Yang, Guanyu Zhang, Ting Yi, Shuran Yang, Shuai Wu, Yongqiang Zhang, Li Zhang, Xi Li, Xiuxuan Wu, Jun Li, Danfeng Yang
文章名称: snRNA-seq reveals subcutaneous white adipose tissue remodeling upon return to thermoneutrality after cold stimulation
杂志: Frontiers in Cell and Developmental Biology
IF: 4.6
发表年份: 2025
发表日期: 2025 May 22
Abstract: [Abstract]
类型: 客户发文
平台: 10X
备注: 材料和方法提及Shbio
关键词: 单核RNA测序，热中性，皮下白色脂肪组织，可塑性，冷刺激
分类: 医学
研究内容: 机制
采样物种分类: 小鼠
通讯作者: Jun Li，Danfeng Yang
单位: 贵州医科大学
区域: 华南

IF 7.9 JOURNAL OF HEADACHE AND PAIN 2025

Selective loss and transcriptional reprogramming of Nox4+ GABAergic neurons in the trigeminal nucleus caudalis of NTG-induced chronic migraine model

产品页

序号: 2874
分类: 试剂
原文: Nuclei were extracted using the Nucleus Isolation Kit (SHBIO, Cat# 52009–10) ac
应用方向: snRNA-seq
产品线: 试剂
服务种类: 细胞核分离试剂盒
样本类型: 组织
样本详情: 脑
作者名: Xu Yunhao, Deng Yiping, Wu Peiyu, Tian Mengke, Liu Chen, Liu Xuyang, Liu Danhua, Guo Yinan, Wang Pengfei, Xu Yuming, Wang Yonggang, Li Yusheng
文章名称: Selective loss and transcriptional reprogramming of Nox4+ GABAergic neurons in the trigeminal nucleus caudalis of NTG-induced chronic migraine model
杂志: JOURNAL OF HEADACHE AND PAIN
IF: 7.9
发表年份: 2025
发表日期: 2025 Nov 18
Abstract: [Abstract]
类型: 客户发文
平台: SeekOne
备注: 材料和方法提及Shbio
关键词: 慢性偏头痛、TNC、GABA能神经元、Nox4、snRNA-seq
分类: 医学
研究内容: 机制
病种: 慢性偏头痛
采样物种分类: 小鼠
通讯作者: Yuming Xu1,3*, Yonggang Wang4* and Yusheng Li
单位: 郑州大学第一附属医院
区域: 华北

IF 15.7 Nature Communications 2025

Fosl2 facilitates chromatin accessibility to determine developmental events during follicular maturation

产品页

序号: 2855
分类: 试剂
原文: nuclei were isolated from 5×104 cells using the efficient nuclear separation kit (SHBIO, 52009).
应用方向: ATAC-seq，CUT&Tag
产品线: 试剂
服务种类: 细胞核分离试剂盒
样本类型: 组织
样本详情: 卵巢
作者名: Zhang Hongyong, Li Zechen, Zhu Yanmei, Lyu Wencong, Wei Wenlu, Wang Haochen, Tian Shuangjie, Yue Wei, Zhong Jiajing, Sun Qing-Yuan, Guan Yiting
文章名称: Fosl2 facilitates chromatin accessibility to determine developmental events during follicular maturation
杂志: Nature Communications
IF: 15.7
发表年份: 2025
发表日期: 2025 Oct 08
Abstract: [Abstract]
类型: 客户发文
平台: 10X
备注: 材料和方法提及Shbio
分类: 医学
研究内容: 机制
病种: 不孕不育
采样物种分类: 小鼠
通讯作者: Qing-Yuan Sun 7 & Yiting Guan
单位: 广东湛江中心人民医院
区域: 华南

IF 15 NEURON 2025

Segregated supramammillary-dentate gyrus circuits modulate cognitive and affective function in healthy and Alzheimer’s disease model mice

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序号: 2853
分类: 单细胞
原文: Shbio Nuclei Isolation Kit Cat# 52009-10
应用方向: snRNA-seq
产品线: 单细胞/试剂
服务种类: 单细胞核转录组测序/细胞核分离试剂盒
使用服务种类: Shbio Nuclei Isolation Kit
样本类型: 组织
样本详情: 下丘脑组织
作者名: Yan-Jia Luo, Longfei Li, Ze-Ka Chen, Ping Dong, Ling Xie, W. Todd Farmer, Qing-Song Yu, Lian-Xiang Jiang, Wei-Kun Su, Hong-Shan Liu, Hua-Zhi Wang, Zhuo-Lei Jiao, David James Melville, Emma Penel, Ryan N. Sheehy, Taylor Landry, Jing Wang, Hong Jiang, Xiao-Hong Xu, Xian Chen, Nan-Jie Xu, Tian Xue, Tian-Le Xu, Ya-Dong Li, Juan Song
文章名称: Segregated supramammillary-dentate gyrus circuits modulate cognitive and affective function in healthy and Alzheimer’s disease model mice
杂志: NEURON
IF: 15
发表年份: 2025
发表日期: 2025 Oct 03
Abstract: [Abstract]
类型: 客户发文
平台: 10X
备注: 材料和方法提及Shbio
分类: 医学
研究内容: 机制
病种: 阿尔兹海默症
采样物种分类: 小鼠
通讯作者: 罗艳佳，李亚东
单位: 上海九院
区域: 华东

IF 2.6 BMC GASTROENTEROLOGY 2025

Elucidating the role of IgA plasma cells and PECAM1-CD38 interaction in intestinal fibrosis: a single-cell RNA sequencing analysis in Crohn's disease

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序号: 2839
分类: 试剂
原文: The nucleus was isolated using a Nucleus Isolation Kit (SHBIO, 52,009–10)
应用方向: snRNA-seq
产品线: 试剂
服务种类: 细胞核分离试剂盒
样本类型: 组织
样本详情: 肠
作者名: Sun Shuyang, Wang Jiheng, Li Kai, Jin Hua, Du Shuwen, Feng Jingyan, Zhang Limin, Li Lei, He Yuqi
文章名称: Elucidating the role of IgA plasma cells and PECAM1-CD38 interaction in intestinal fibrosis: a single-cell RNA sequencing analysis in Crohn's disease
杂志: BMC GASTROENTEROLOGY
IF: 2.6
发表年份: 2025
发表日期: 2025 Oct 09
Abstract: [Abstract]
类型: 客户发文
平台: SeekOne
备注: 材料和方法提及Shbio
关键词: 肠纤维化、克罗恩病、溃疡性结肠炎、单细胞RNA测序、IgA浆细胞、炎症相关成纤维细胞、PECAM1、CD38、IL-1β
分类: 医学
研究内容: 机制
病种: 肠纤维化、克罗恩病、溃疡性结肠炎
采样物种分类: 人
通讯作者: Lei Li，Yuqi He
单位: 首都医科大学北京胸科医院
区域: 华北

IF 10.1 International Journal of Surgery 2025

Neuron-specific transcriptomic dysregulation in methotrexate-induced cognitive impairment revealed by SnRNA-seq

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序号: 2829
分类: 单细胞
原文: Boyou® Cell Nucleus Isolation Kit (SHBIO, 52009-10, China), a
应用方向: snRNA-seq
产品线: 试剂/单细胞
服务种类: 细胞核分离盒，单细胞核转录组测序
样本类型: 组织
样本详情: 海马
作者名: Ye Jishi, Ding Yu, Wu Ruolan, Ding Huang, Ren Juan, Xia Zhongyuan, Chen Jingli, Xie Shuang, Jia Yifan
文章名称: Neuron-specific transcriptomic dysregulation in methotrexate-induced cognitive impairment revealed by SnRNA-seq
杂志: International Journal of Surgery
IF: 10.1
发表年份: 2025
发表日期: 2025 Sep 24
Abstract: [Abstract]
类型: 客户发文
平台: 10X
备注: 材料和方法提及Shbio
关键词: 甲氨蝶呤、单核RNA测序、海马、神经元、认知障碍
分类: 医学
研究内容: 机制
病种: 认知障碍
采样物种分类: 小鼠
通讯作者: Yifan Jia
单位: 武汉仁济医院
区域: 华南

IF 10.1 MOLECULAR PSYCHIATRY 2025

Bcl11a deficiency in cerebellar Purkinje cells causes ataxia and autistic-like behavior by altering Vav3

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序号: 2812
分类: 单细胞
原文: The nucleus was isolated using the Shbio Nuclei Isolation Kit (52009-10, SHBIO).
应用方向: snRNA-seq
产品线: 单细胞/试剂
服务种类: 单细胞核转录组测序/细胞核分离试剂盒
使用服务种类: Shbio Nuclei Isolation Kit
样本类型: 组织
样本详情: 小脑
作者名: Zhang Jie, Li Yuan-Xin, Huang Qian, Yuan Yu, Chen Jun-Yang, Yang Fu-Wei, Yang Lin, Liu Lin-Yun, Yu Yong-Chun
文章名称: Bcl11a deficiency in cerebellar Purkinje cells causes ataxia and autistic-like behavior by altering Vav3
杂志: MOLECULAR PSYCHIATRY
IF: 10.1
发表年份: 2025
发表日期: 2025 Aug 25
Abstract: [Abstract]
类型: 客户发文
平台: 10X
备注: 材料和方法提及Shbio
分类: 医学
研究内容: 机制
病种: 小脑功能障碍
采样物种分类: 小鼠
通讯作者: Lin-Yun Liu ， Yong-Chun Yu
单位: 上海静安中心医院脑科学研究所
区域: 华东

IF 7.5 Journal of Translational Medicine 2025

A multi-omic single-cell landscape reveals transcription and epigenetic regulatory features of t(8;21) AML

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序号: 2785
分类: 单细胞
原文: The nuclei were isolated with a Shbio Cell Nuclear Isolation Kit (Shbio, #52009–10).
应用方向: scATAC-seq
产品线: 单细胞/试剂
服务种类: 单细胞转录组/VDJ/ATAC测序/细胞核分离试剂盒
使用服务种类: Shbio Cell Nuclear Isolation Kit (Shbio, #52009–10).
样本类型: 组织
样本详情: 骨髓
作者名: Li Xue-Ping, Gao Yan, Zhao Bai-Tian, Dai Yu-Ting, Mao Jia-Ying, Liang Yang, Jiang Lu
文章名称: A multi-omic single-cell landscape reveals transcription and epigenetic regulatory features of t(8;21) AML
杂志: Journal of Translational Medicine
IF: 7.5
发表年份: 2025
发表日期: 2025 Jul 24
Abstract: [Abstract]
类型: 客户发文
平台: 10X
备注: 致谢提及Shanghai Biotechnology Corporation，罗丽芳
关键词: scRNA-seq、scATAC-seq、TCR库、T（8；21）AML
分类: 医学
研究内容: 机制
病种: 急性髓性白血病
采样物种分类: 人
通讯作者: 李雪萍
单位: 中山大学附属肿瘤医院
区域: 华南

IF 48.5 NATURE 2025

Constitutively active glucagon receptor drives high blood glucose in birds

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序号: 2770
分类: 试剂
原文: Nuclei were isolated using a Nucleus Isolation Kit (SHBIO 52009-10) a
应用方向: snRNA-seq
产品线: 试剂
服务种类: 细胞核分离试剂盒
使用服务种类: Nucleus Isolation Kit (SHBIO 52009-10)
样本类型: 组织
样本详情: 肝脏
作者名: Zhang Chang, Xiang Xiangying, Liu Jian, Huang Yongjie, Xue Jingwen, Sun Qian, Leng Song, Liu Shaobo, He Xuefei, Hu Peng, Zhan Xiangjiang, Qiu Qiang, Yang Shilong, Brosius Jürgen, Deng Cheng
文章名称: Constitutively active glucagon receptor drives high blood glucose in birds
杂志: NATURE
IF: 48.5
发表年份: 2025
发表日期: 2025 Mar 03
Abstract: [Abstract]
类型: 客户发文
平台: SeekGene
备注: 材料和方法提及Shbio，服务不是伯豪做的
分类: 动物
研究内容: 机制
采样物种分类: 鸡
通讯作者: Cheng Deng
单位: 华西医院
区域: 华南

IF 9.3 ACTA NEUROPATHOLOGICA 2025

Multiplatform molecular analyses reveal two molecular subgroups of NF2-related schwannomatosis vestibular schwannomas with distinct tumour microenvironment and therapeutic vulnerabilities

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序号: 2739
分类: 单细胞
原文: Nuclei were isolated from each sample using a Nucleus Isolation Kit (Shbio, #52,009-10, Shanghai, China) a
应用方向: snRNA-seq
产品线: 单细胞/试剂
服务种类: 单细胞核转录组测序/细胞核分离试剂盒
样本类型: 组织
样本详情: 神经鞘瘤
作者名: Zhao Fu, Teng Xu-Fei, Zhang Jing, Li Shi-Wei, Wang Lei-Ming, Zhao Han-Guang, Zhang Shun, Zhao Chi, Li Peng, Zhao Xiao-Bin, Song Shu-Hui, Liu Pi-Nan
文章名称: Multiplatform molecular analyses reveal two molecular subgroups of NF2-related schwannomatosis vestibular schwannomas with distinct tumour microenvironment and therapeutic vulnerabilities
杂志: ACTA NEUROPATHOLOGICA
IF: 9.3
发表年份: 2025
发表日期: 2025 May 09
Abstract: [Abstract]
类型: 客户发文
平台: 10x
备注: 材料和方法提及Shbio
关键词: 前庭神经鞘瘤·NF2相关神经鞘瘤病·分子亚群·巨噬细胞·肿瘤微环境
分类: 医学
研究内容: 机制
病种: 神经鞘瘤病
采样物种分类: 人
通讯作者: Fu Zhao，Shu‑Hui Song，Pi‑Nan Liu
单位: 北京神经外科研究所
区域: 华北

IF 6.2 ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 2025

Characterizing microglial heterogeneity in autophagy impairment of Paraquat-induced Parkinson’s disease-like neurodegeneration

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序号: 2734
分类: 试剂
原文: for single nuclei isolation, we employed the Shbio Cell Nuclear Isolation Kit (Shbio, #52009–10, Shanghai, China)
应用方向: snRNA-seq
产品线: 试剂
服务种类: 细胞核分离试剂盒
样本类型: 组织
样本详情: 脑
作者名: Yu Zhang, Yihua Jiang, Zhen Yu, Yinhan Li, Zhiyu Zhang, Fuli Zheng, Hong Hu, Guangxia Yu, Zhenkun Guo, Siying Wu, Wenya Shao, Huangyuan Li
文章名称: Characterizing microglial heterogeneity in autophagy impairment of Paraquat-induced Parkinson’s disease-like neurodegeneration
杂志: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
IF: 6.2
发表年份: 2025
发表日期: 2025 May 21
Abstract: [Abstract]
类型: 客户发文
平台: 10x
备注: 材料和方法提及Shbio
关键词: 单细胞转录组学帕金森病自噬小胶质细胞异质性
分类: 疾病
研究内容: 机制
病种: 帕金森病
采样物种分类: 小鼠
通讯作者: Huangyuan Li
单位: 福建医科大学
区域: 华南

IF 20.5 Nature Microbiology 2025

Integrating microbial GWAS and single-cell transcriptomics reveals associations between host cell populations and the gut microbiome

产品页

序号: 2720
分类: 试剂
原文: he nuclei from mouse liver tissues were isolated using the Nucleus Isolation kit (SHBIO, 52009-10)
应用方向: snRNA-seq
产品线: 试剂
服务种类: 细胞核分离试剂盒
使用服务种类: Nucleus Isolation kit (SHBIO, 52009-10)
样本类型: 组织
样本详情: 肝脏
作者名: Li Jingjing, Ma Yunlong, Cao Yue, Zheng Gongwei, Ren Qing, Chen Cheng, Zhu Qunyan, Zhou Yijun, Lu Yu, Zhang Yaru, Deng Chunyu, Chen Wei-Hua, Su Jianzhong
文章名称: Integrating microbial GWAS and single-cell transcriptomics reveals associations between host cell populations and the gut microbiome
杂志: Nature Microbiology
IF: 20.5
发表年份: 2025
发表日期: 2025 Apr 07
Abstract: [Abstract]
类型: 客户发文
平台: SeekGene
备注: 材料和方法提及Shbio
分类: 微生物
研究内容: 机制
采样物种分类: 小鼠
通讯作者: Jianzhong Su
单位: 温州医科大学
区域: 华东

IF 6.1 Journal of Translational Medicine 2025

scRNA-seq reveals that VEGF signaling mediates the response to neoadjuvant anlotinib combined with PD-1 blockade therapy in non-small cell lung cancer

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序号: 2719
分类: 单细胞
原文: The Isolation Kit furnished by Shanghai Biotechnology Corporation was exploited. Nuclear suspensions were obtained in accordance with the standard protocols of the kit.
应用方向: snRNA-seq
产品线: 单细胞/试剂
服务种类: 单细胞核转录组测序/细胞核分离试剂盒
样本类型: 组织
样本详情: 肺
作者名: Huang Ziqi, Li Li, Zhao Xiaohe, Jin Haixia, Shen Meng, Li Baihui, Zeng Yu, Zhang Qinfen, Wang Qiyu, Wang Meng, Yang Lili
文章名称: scRNA-seq reveals that VEGF signaling mediates the response to neoadjuvant anlotinib combined with PD-1 blockade therapy in non-small cell lung cancer
杂志: Journal of Translational Medicine
IF: 6.1
发表年份: 2025
发表日期: 2025 Apr 25
Abstract: [Abstract]
类型: 客户发文
平台: 10X
备注: 材料和方法提及Shanghai Biotechnology Corporation
关键词: 新辅助免疫治疗、安洛替尼、PD-1阻断、非小细胞肺癌、肿瘤微环境、单细胞、VEGFR、PLA2G4A
分类: 疾病
研究内容: 机制
病种: 非小细胞肺癌
采样物种分类: 人
通讯作者: Lili Yang1*
单位: 天津医科大学
区域: 华北

IF 7.9 Clinical and Translational Medicine 2025

Proteogenomic characterisation of primary oral cancer unveils extracellular matrix remodelling and immunosuppressive microenvironment linked to lymph node metastasis

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序号: 2709
分类: 单细胞
原文: Nuclei were isolated using the Shbio Nuclei Isolation Kit (SHBIO,#52009-10,China)
应用方向: snRNA-seq
产品线: 单细胞空转/试剂
服务种类: 单细胞核转录组测序/空间转录组测序细胞核分离试剂盒
样本类型: 组织/血液
样本详情: 肿瘤组织/血液
作者名: Yu Liu, Zhenyu Yang, Jingya Jane Pu, Jie Zhong, Ui-Soon Khoo, Yu-Xiong Su, Gao Zhang
文章名称: Proteogenomic characterisation of primary oral cancer unveils extracellular matrix remodelling and immunosuppressive microenvironment linked to lymph node metastasis
杂志: Clinical and Translational Medicine
IF: 7.9
发表年份: 2025
发表日期: 2025 Mar 04
Abstract: [Abstract]
类型: 客户发文
平台: 10x
备注: 材料和方法提及SHBIO
关键词: 淋巴结转移，口腔鳞状细胞癌，单细胞分析
分类: 医学
研究内容: 机制
病种: 口腔鳞状细胞癌
采样物种分类: 人
通讯作者: Gao Zhang
单位: 香港中文大学
区域: 华南

IF 21.2 NATURE NEUROSCIENCE 2025

Mitochondrial respiratory complex IV deficiency recapitulates amyotrophic lateral sclerosis

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序号: 2706
分类: 试剂
原文: Nuclei from frozen anterior horns of lumbar spinal cord tissues were isolated using the Shbio Nuclei Isolation Kit (SHBIO).
应用方向: snRNA-seq
产品线: 试剂
服务种类: 细胞核分离试剂盒
使用服务种类: Shbio Nuclei Isolation Kit (SHBIO).
样本类型: 组织
样本详情: 腰椎脊髓
作者名: Cheng Man, Lu Dan, Li Kexin, Wang Yan, Tong Xiwen, Qi Xiaolong, Yan Chuanzhu, Ji Kunqian, Wang Junlin, Wang Wei, Lv Huijiao, Zhang Xu, Kong Weining, Zhang Jian, Ma Jiaxin, Li Keru, Wang Yaheng, Feng Jingyu, Wei Panpan, Li Qiushuang, Shen Chengyong, Fu Xiang-Dong, Ma Yuanwu, Zhang Xiaorong
文章名称: Mitochondrial respiratory complex IV deficiency recapitulates amyotrophic lateral sclerosis
杂志: NATURE NEUROSCIENCE
IF: 21.2
发表年份: 2025
发表日期: 2025 Mar 11
Abstract: [Abstract]
类型: 客户发文
平台: MobiDrop
备注: 材料和方法提及SHBIO
分类: 医学
研究内容: 机制
病种: 肌萎缩侧索硬化症
采样物种分类: 大鼠
通讯作者: Yuanwu Ma,Xiaorong Zhang
单位: 北京协和医院
区域: 华北

IF 3.9 Frontiers in Endocrinology 2025

Single-nucleus RNA sequencing reveals dynamic changes in the microenvironment of visceral adipose tissue and metabolic characteristics after cold exposure

产品页

序号: 2696
分类: 单细胞
原文: Nuclei were extracted using the BioYoo Nuclear IsolationKit (SHBIO, #52009-10, Shanghai, China)
应用方向: snRNA-seq
产品线: 单细胞/试剂
服务种类: 单细胞核转录组测序/细胞核分离试剂盒
使用服务种类: BioYoo Nuclear Isolation Kit (SHBIO, #52009-10,
样本类型: 组织
样本详情: 附睾白色脂肪组织
作者名: Ting Yi, Shuai Wu, Yusha Yang, Xi Li, Shuran Yang, Yongqiang Zhang, Li Zhang, Yuyu Hu, Guanyu Zhang, Jun Li, Danfeng Yang
文章名称: Single-nucleus RNA sequencing reveals dynamic changes in the microenvironment of visceral adipose tissue and metabolic characteristics after cold exposure
杂志: Frontiers in Endocrinology
IF: 3.9
发表年份: 2025
发表日期: 2025 Mar 24
Abstract: [Abstract]
类型: 客户发文
平台: 10X
备注: 材料和方法提及SHBIO
关键词: 冷暴露、内脏脂肪组织、snRNA-seq、代谢稳态、细胞异质性
分类: 医学
研究内容: 机制
病种: 代谢疾病
采样物种分类: 小鼠
通讯作者: Jun Li1* and Danfeng Yang
单位: 贵州医科大学
区域: 华南

IF 14.3 Advanced Science 2025

Radiotherapy-Associated Cellular Senescence and EMT Alterations Contribute to Distinct Disease Relapse Patterns in Locally Advanced Cervical Cancer

产品页

序号: 2689
分类: 单细胞
原文: The samples were mechanically dissociated with the Shbio Cell Nuclear Isolation Kit (Shbio, 52009–10, Shanghai, China)
应用方向: snRNA-seq
产品线: 单细胞/试剂
服务种类: 单细胞核转录组测序/细胞核分离试剂盒
样本类型: 组织
样本详情: 宫颈
作者名: Lei Zhang, Jun Ma, Jun Zhang, Minjie Hu, Jinlin Cheng, Bin Hu, Junjun Zhou, Di Zhou, Yongrui Bai, Xiumei Ma, Jianming Tang, Haiyan Chen, Ying Jing
文章名称: Radiotherapy-Associated Cellular Senescence and EMT Alterations Contribute to Distinct Disease Relapse Patterns in Locally Advanced Cervical Cancer
杂志: Advanced Science
IF: 14.3
发表年份: 2025
发表日期: 2025 Feb 04
Abstract: [Abstract]
类型: 客户发文
平台: 10x
备注: 材料和方法提及Shanghai Biotechnology Corporation
分类: 医学
研究内容: 机制
病种: 宫颈癌
采样物种分类: 人
通讯作者: Haiyan Chen,Xiumei Ma
单位: 上海仁济医院
区域: 华东

IF 7.7 INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 2025

Single-nucleus RNA sequencing decodes abnormal cell-collagen communication in a sheep endometrial fibrosis model

产品页

序号: 2683
分类: 试剂
原文: Following to the instructions provided with the Shbio Cell Nuclear Isolation Kit (Burghardt, 52,009–10, Shanghai)
应用方向: snRNA-seq
产品线: 试剂
服务种类: 细胞核分离试剂盒
样本类型: 组织
样本详情: 子宫内膜
作者名: Tingting Chu, Jiashun Tong, Zhongshi Zhu, Guoliang Zhang, Yunan Weng, Lei Sun, Le Sun, Jiuzeng Cui, Jiaxin Liu, Yuhang Xiao, Lei Zhang, Yuxuan Song
文章名称: Single-nucleus RNA sequencing decodes abnormal cell-collagen communication in a sheep endometrial fibrosis model
杂志: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
IF: 7.7
发表年份: 2025
发表日期: 2025 Feb 05
Abstract: [Abstract]
类型: 客户发文
备注: 材料和方法提及Shbio
分类: 动物
研究内容: 机制
采样物种分类: 绵羊
通讯作者: Yuxuan Song
单位: 西北农林
区域: 华西

IF 6.1 Journal of Translational Medicine 2025

Single-cell atlas reveals multi-faced responses of losartan on tubular mitochondria in diabetic kidney disease

产品页

序号: 2665
分类: 单细胞
原文: Cell nucleus separation uses a cell nucleus separation kit (Shanghai biotechnology corporation, 52009-10).
应用方向: snRNA-seq
产品线: 单细胞/试剂
服务种类: 细胞核转录组测序/细胞核分离试剂盒
样本类型: 组织
样本详情: 肾
作者名: Zhu Zhen, Luan Guangxin, Wu Song, Song Yiyi, Shen Shuang, Wu Kaiyue, Qian Shengnan, Jia Weiping, Yin Jun, Ren Tao, Ye Jianping, Wei Li
文章名称: Single-cell atlas reveals multi-faced responses of losartan on tubular mitochondria in diabetic kidney disease
杂志: Journal of Translational Medicine
IF: 6.1
发表年份: 2025
发表日期: 2025 Jau 21
Abstract: [Abstract]
类型: 客户发文
平台: 10x
备注: 材料和方法提及Shanghai Biotechnology Corporation
关键词: snRNA-seq、线粒体吞噬、线粒体稳态紊乱、糖尿病肾病、氯沙坦
分类: 疾病
研究内容: 机制
病种: 糖尿病肾病
采样物种分类: 小鼠
通讯作者: 李巍
单位: 上海交通大学附属六院
区域: 华东

IF 1.3 STAR Protocols 2025

Protocol for the isolation of silk glands from silkworms for snRNA-seq and spatial transcriptomics

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序号: 2651
分类: 试剂
原文: Nucleusisolationkit，SHBIO， Cat#52009-10
应用方向: snRNA-seq
产品线: 试剂
服务种类: 细胞核分离试剂盒
样本类型: 组织
样本详情: 丝腺
作者名: Yan Ma, Keshu Dong, Jie Hu, Yiyun Tang, Hanfu Xu
文章名称: Protocol for the isolation of silk glands from silkworms for snRNA-seq and spatial transcriptomics
杂志: STAR Protocols
IF: 1.3
发表年份: 2025
发表日期: 2025 Jau 12
Abstract: [Abstract]
类型: 客户发文
备注: 材料和方法提及Shbio
分类: 动物
研究内容: 基础
采样物种分类: 家蚕
通讯作者: Hanfu Xu
单位: 西南大学
区域: 华南

IF 14.3 Advanced Science 2024

Neuroligin 1 Regulates Autistic-Like Repetitive Behavior through Modulating the Activity of Striatal D2 Receptor-Expressing Medium Spiny Neurons

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序号: 2648
分类: 单细胞
原文: The nucleus was isolated using the Shbio Nuclei Isolation Kit (SHBIO, #52009-10, China).
应用方向: snRNA-seq
产品线: 单细胞/试剂
服务种类: 单细胞核转录组测序/细胞核分离试剂盒
样本类型: 组织
样本详情: 纹状体
作者名: Dandan Lv, An Liu, Ziyue Yi, Mingdao Mu, Miao Wu, Xingcan Li, Kun Cao, Ruining Liu, Zhengping Jia, Junhai Han, Wei Xie
文章名称: Neuroligin 1 Regulates Autistic-Like Repetitive Behavior through Modulating the Activity of Striatal D2 Receptor-Expressing Medium Spiny Neurons
杂志: Advanced Science
IF: 14.3
发表年份: 2024
发表日期: 2024 Dec 11
Abstract: [Abstract]
类型: 客户发文
平台: 10X
备注: 材料和方法提及Shbio
关键词: 自闭症谱系障碍（ASD）、D2-MSN、神经原1、限制性和重复性行为（RRB）、纹状体
分类: 疾病
研究内容: 机制
病种: 自闭症谱系障碍（ASD）
采样物种分类: 小鼠
通讯作者: An Liu，Wei Xie
单位: 东南大学
区域: 华东

IF 9.4 PNAS 2024

Pathogenic mechanism and therapeutic intervention of impaired N7-methylguanosine (m7G) tRNA modification

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序号: 2630
分类: 单细胞
原文: cell nuclei were isolated the from brain tissues using Shbio Cell Nuclear Isolation Kit (Shbio, #52009–10).
应用方向: snRNA-seq
产品线: 单细胞/试剂
服务种类: 单细胞核转录组测序/细胞核分离试剂盒
样本类型: 组织
样本详情: 脑
作者名: Jieyi Ma, Siyi Zheng, Chenrui An, Hui Han, Qiwen Li, Ying Huang, Gan Xiong, Shuang Chen, Siyao Guo, Zhaoyu Wang, Wei Wei, Yudan Shang, Yushan Ji, Cuiyun Yang, Junho Choe, Quan Yuan, Yong Fan, Canfeng Zhang , and Shuibin Lin
文章名称: Pathogenic mechanism and therapeutic intervention of impaired N7-methylguanosine (m7G) tRNA modification
杂志: PNAS
IF: 9.4
发表年份: 2024
发表日期: 2024 Oct 29
Abstract: [Abstract]
类型: 客户发文
平台: 10X
备注: 附录材料和方法提及Shanghai Biotechnology Corporation
关键词: N7-甲基鸟苷（m7G）|tRNA修饰|WDR4|基因治疗|神经疾病
分类: 疾病
研究内容: 机制
病种: 神经疾病
采样物种分类: 小鼠
通讯作者: Yong Fanc, Canfeng Zhangb, and Shuibin Lin
单位: 中山大学附属第一医院
区域: 华南

IF 6.2 ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 2024

Brain single-cell transcriptomics highlights comorbidity-related cell type-specific changes of Parkinson’s disease with major depressive disorder after paraquat exposure

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序号: 2601
分类: 试剂
原文: We isolated single nuclei using a Shbio Cell Nuclear Isolation Kit (Shbio, #52009–10, Shanghai, China)
应用方向: snRNA-seq
产品线: 试剂
服务种类: 细胞核分离试剂盒
样本类型: 组织
样本详情: 脑
作者名: Yu Zhang, Yihua Jiang, Yinhan Li, Zhen Yu, Xinpei Lin, Fuli Zheng, Hong Hu, Wenya Shao, Guangxia Yu, Zhenkun Guo, Siying Wu, Huangyuan Li
文章名称: Brain single-cell transcriptomics highlights comorbidity-related cell type-specific changes of Parkinson’s disease with major depressive disorder after paraquat exposure
杂志: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
IF: 6.2
发表年份: 2024
发表日期: 2024 Oct 16
Abstract: [Abstract]
类型: 客户发文
备注: 材料和方法提及Shbio
关键词: 单细胞RNA测序共病帕金森病重度抑郁症百草枯
分类: 疾病
研究内容: 机制
病种: 帕金森，重度抑郁
采样物种分类: 小鼠
通讯作者: Siying Wu，Huangyuan Li
单位: 福建医科大学
区域: 华南

IF 7.5 Cell Reports 2024

RAC1 inhibition ameliorates IBSP-induced bone metastasis in lung adenocarcinoma

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序号: 2551
分类: 单细胞
原文: Nuclei were isolated using the Nuclei Isolation Kit (SHBIO, CAT No.52009-10)
应用方向: snRNA-seq
产品线: 试剂/单细胞
服务种类: 细胞核分离试剂盒/单细胞核转录组测序
样本类型: 组织
样本详情: 骨转移样本
作者名: Xiaoshen Zhang, Xijun Liang, Yaokai Wen, Fengying Wu, Guanghui Gao, Lei Zhang, Yifeng Gu, Jianping Zhang, Fei Zhou, Wei Li, Liang Tang, Xiaojun Yang, Hui Zhao, Caicun Zhou, Fred R. Hirsch
文章名称: RAC1 inhibition ameliorates IBSP-induced bone metastasis in lung adenocarcinoma
杂志: Cell Reports
IF: 7.5
发表年份: 2024
发表日期: 2024 Jul 24
Abstract: [Abstract]
类型: 客户发文
平台: 10x
备注: 材料和方法提及Shbio
分类: 疾病
研究内容: 机制
病种: 肺腺癌
采样物种分类: 人
通讯作者: 周彩存
单位: 上海肺科医院
区域: 华东

IF 7.7 INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 2024

GSK3 regulation Wnt/β-catenin signaling affects adipogenesis in bovine skeletal muscle fibro/adipogenic progenitors

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序号: 2544
分类: 试剂
原文: homogenized in Nuclei EZ Lysis Buffer (52009-10, Shbio, China)
应用方向: snRNA-seq
产品线: 试剂
服务种类: 细胞核分离试剂盒
样本类型: 组织
样本详情: 骨骼肌组织
作者名: Junfang Zhang, Enze Wang, Qiang Li, Yinghua Peng, Huaina Jin, Sajida Naseem, Bin Sun, Sungkwon Park, Seongho Choi, Xiangzi Li
文章名称: GSK3 regulation Wnt/β-catenin signaling affects adipogenesis in bovine skeletal muscle fibro/adipogenic progenitors
杂志: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
IF: 7.7
发表年份: 2024
发表日期: 2024 Jul 04
Abstract: [Abstract]
类型: 客户发文
备注: 材料和方法提及Shbio,单细胞测序博奥做的
关键词: 单核RNA测序，Wnt/β-catenin信号糖原合酶激酶3成纤维/脂肪祖细胞肌内脂肪
分类: 动物
研究内容: 机制
采样物种分类: 延边牛
通讯作者: Xiangzi Li
单位: 延边大学
区域: 华北

IF 7.5 Cell Reports 2024

The architecture of silk-secreting organs during the final larval stage of silkworms revealed by single-nucleus and spatial transcriptomics

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序号: 2543
分类: 试剂
原文: The nuclei of the SG sampleswere isolated using a Nucleus Isolation Kit (SHBIO 52009-10)
应用方向: snRNA-seq
产品线: 试剂
服务种类: 细胞核分离试剂盒
样本类型: 组织
样本详情: 丝腺
作者名: Yan Ma, Qingjun Li, Yiyun Tang, Zhiyong Zhang, Rongpeng Liu, Qin Luo, Yuting Wang, Jie Hu, Yuqin Chen, Zhiwei Li, Chen Zhao, Yiting Ran, Yuanyuan Mu, Yinghao Li, Xiaoqing Xu, Yuyan Gong, Zihan He, Yongbing Ba, Kaiqi Guo, Keshu Dong, Xiao Li, Wei Tan, Yumeng Zhu, Zhonghuai Xiang, Hanfu Xu
文章名称: The architecture of silk-secreting organs during the final larval stage of silkworms revealed by single-nucleus and spatial transcriptomics
杂志: Cell Reports
IF: 7.5
发表年份: 2024
发表日期: 2024 Jul 10
Abstract: [Abstract]
类型: 客户发文
备注: 材料和方法提及Shanghai Biotechnology Corporation
分类: 动物
研究内容: 机制
采样物种分类: 家蚕
通讯作者: Hanfu Xu
单位: 西南大学
区域: 华南

IF 6.1 Neural Regeneration Research 2024

Spatial transcriptomics combined with single-nucleus RNA sequencing reveals glial cell heterogeneity in the human spinal cord

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序号: 2523
分类: 试剂
原文: Cell nuclei were isolated using a Nucleus Isolation Kit (No. 52009-10; SHBIO, Shanghai, China),
应用方向: snRNA-seq
产品线: 试剂
服务种类: 细胞核分离试剂盒
样本类型: 组织
样本详情: 脊髓
作者名: Chen Yali, Wei Yiyong, Liu Jin, Zhu Tao, Zhou Cheng, Zhang Donghang
文章名称: Spatial transcriptomics combined with single-nucleus RNA sequencing reveals glial cell heterogeneity in the human spinal cord
杂志: Neural Regeneration Research
IF: 6.1
发表年份: 2024
发表日期: 2024 Jun 26
Abstract: [Abstract]
类型: 客户发文
备注: 材料和方法提及Shbio，博奥服务
关键词: 星形胶质细胞；胶质细胞；人类小胶质细胞；少突胶质细胞；性别分化；单核RNA测序；空间转录组学；物种变异；脊髓
分类: 疾病
研究内容: 机制
病种: 脊髓相关疾病
采样物种分类: 人
通讯作者: Donghang Zhang
单位: 四川华西医院
区域: 华南

IF 4.8 Frontiers in Aging Neuroscience 2024

Single-cell sequencing of the substantia nigra reveals microglial activation in a model of MPTP

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序号: 2519
分类: 单细胞
原文: nuclei isolated according to the nuclear isolation by snRNA-seq protocol of 10× Genomics (Shbio, #52009–10, Shanghai, China).
应用方向: snRNA-seq
产品线: 试剂/单细胞
服务种类: 细胞核分离试剂盒/单细胞核转录组测序
样本类型: 组织
样本详情: 脑
作者名: Liu Qing, Liu Ziyu, Xie Wenmeng, Li Yibo, Wang Hongfang, Zhang Sanbing, Wang Wenyu, Hao Jiaxin, Geng Dandan, Yang Jing, Wang Lei
文章名称: Single-cell sequencing of the substantia nigra reveals microglial activation in a model of MPTP
杂志: Frontiers in Aging Neuroscience
IF: 4.8
发表年份: 2024
发表日期: 2024 Jun 17
Abstract: [Abstract]
类型: 客户发文
平台: 10X
备注: 材料和方法提及Shanghai Biotechnology Corporation
关键词: 帕金森病、单核测序、小胶质细胞、细胞-细胞通讯、细胞状态
分类: 疾病
研究内容: 机制
病种: 帕金森病
采样物种分类: 小鼠
通讯作者: Dandan Geng， Jing Yang，Lei Wang
单位: 河北医科大学
区域: 华北

IF 7.7 COMPUTERS IN BIOLOGY AND MEDICINE 2024

Dissecting microenvironment in cystadenomas and hepatic cysts based on single nucleus RNA-sequencing data

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序号: 2501
分类: 单细胞
原文: Cell nuclei were isolated using Shbio Nucleic separation Kit (SHBIO, #52009-10, China).
应用方向: snRNA-seq
产品线: 试剂/单细胞
服务种类: 细胞核分离试剂盒/单细胞转录组测序
使用服务种类: Shbio Nucleic separation Kit
样本类型: 组织
样本详情: 肝囊肿
作者名: Congxue Hu, Yongqi Lei, Xinyang Liu, Xingxin Yu, Zhida Geng, Yu Liu, Liyu Yang, Xuehong Tie, Wenzhe Zhou, Xia Li, Yunpeng Zhang, Yingjian Liang
文章名称: Dissecting microenvironment in cystadenomas and hepatic cysts based on single nucleus RNA-sequencing data
杂志: COMPUTERS IN BIOLOGY AND MEDICINE
IF: 7.7
发表年份: 2024
发表日期: 2024 May 03
Abstract: [Abstract]
类型: 客户发文
平台: 10X
备注: 材料和方法提及Shbio
关键词: 单核RNA测序空间转录组学测序微环境囊腺瘤肝囊肿
分类: 疾病
研究内容: 机制
病种: 囊腺瘤肝囊肿
采样物种分类: 人
通讯作者: Yunpeng Zhang，Yingjian Liang
单位: 哈尔滨医科大学第一附属医院
区域: 华北

IF 3.5 GENE 2024

Mapping cell diversity in human sporadic cerebral cavernous malformations

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序号: 2492
分类: 单细胞
原文: We thank Shanghai Biotechnology Corporation
应用方向: snRNA-seq& Bulk ATAC-seq
产品线: 单细胞/表观
服务种类: 单细胞核RNA测序/ATAC
样本类型: 组织
样本详情: 血管组织
作者名: Xiaocan Hou, Feng Liang, Jiaoxing Li, Yibing Yang, Chuhuai Wang, Tiewei Qi, Wenli Sheng
文章名称: Mapping cell diversity in human sporadic cerebral cavernous malformations
杂志: GENE
IF: 3.5
发表年份: 2024
发表日期: 2024 May 22
Abstract: [Abstract]
类型: 客户发文
备注: 致谢提及Shanghai Biotechnology Corporation
关键词: CCM 单细胞RNA测序内皮细胞平滑肌细胞 PVT1
分类: 疾病
病种: 脑海绵状畸形
采样物种分类: 人
通讯作者: Wenli Sheng
单位: 中山大学附属第一医院
区域: 华南

IF 3.4 Molecular Medicine Reports 2024

Network pharmacology combined with experimental validation to investigate the effect of Rongjin Niantong Fang on chondrocyte apoptosis in knee osteoarthritis

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序号: 2480
分类: 试剂
原文: Nuclear and Cytoplasmic Protein Extraction Kit used were obtained from Shanghai Biotechnology Co., Ltd
应用方向: snRNA-seq
产品线: 试剂
服务种类: 细胞核分离试剂盒
样本类型: 组织
样本详情: 软骨
作者名: Jun Chen, Ting Zhang, Qingqing Luo, Ruyi Wang, Yuting Dai, Zhenyuan Chen, Chutian Zhang, Xuzheng Chen, Guangwen Wu
文章名称: Network pharmacology combined with experimental validation to investigate the effect of Rongjin Niantong Fang on chondrocyte apoptosis in knee osteoarthritis
杂志: Molecular Medicine Reports
IF: 3.4
发表年份: 2024
发表日期: 2024 Apr 15
Abstract: [Abstract]
类型: 客户发文
备注: 材料和方法提及Shanghai Biotechnology Corporation
关键词: 膝骨关节炎，荣筋念痛方，PARP1/AIF通路，细胞凋亡，网络药理学
分类: 疾病
研究内容: 机制
病种: 膝骨关节炎
采样物种分类: 大鼠
通讯作者: Guangwen Wu
单位: 福建中医药大学
区域: 华南

IF 6.8 ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 2024

Single-cell RNA-sequencing of cellular heterogeneity and pathogenic mechanisms in paraquat-induced Parkinson's disease with depression

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序号: 2473
分类: 试剂
原文: Nuclei were then separated using a Shbio Cell Nuclear Isolation Kit (Shbio, #52009–10, Shanghai, China)
应用方向: snRNA-seq
产品线: 试剂
服务种类: 细胞核分离试剂盒
使用服务种类: Nuclear Isolation Kit(Shbio, #52009–10）
样本类型: 组织
样本详情: 脑
作者名: Yali Weng, Yu Zhang, Yinhan Li, Xinpei Lin, Zhenkun Guo, Hong Hu, Wenya Shao, Guangxia Yu, Fuli Zheng, Ping Cai, Huangyuan Li, Siying Wu
文章名称: Single-cell RNA-sequencing of cellular heterogeneity and pathogenic mechanisms in paraquat-induced Parkinson's disease with depression
杂志: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
IF: 6.8
发表年份: 2024
发表日期: 2024 Mar 05
Abstract: [Abstract]
类型: 客户发文
备注: 材料和方法提及Shanghai Biotechnology Corporation
关键词: 帕金森病抑郁症，单细胞RNA测序，细胞异质性，谷氨酸能簇
分类: 疾病
研究内容: 机制
病种: 帕金森病抑郁症
采样物种分类: 小鼠
通讯作者: Huangyuan Li，Siying Wu
单位: 福建医科大学
区域: 华南

IF 12.4 Theranostics 2024

An advanced comprehensive muti-cell-type-specific model for predicting anti-PD-1 therapeutic effect in melanoma

产品页

序号: 2458
分类: 单细胞
原文: Nuclei were isolated using the ShBio Nuclei Isolation Kit (SHBIO, #52009-10, Shanghai, China).
应用方向: snRNA-seq
产品线: 试剂/单细胞
服务种类: 抽核试剂/单细胞核转录组测序
使用服务种类: ShBio Nuclei Isolation Kit
样本类型: 组织
样本详情: 骨软组织
作者名: Wei Sun, Yu Zhu, Zijian Zou, Lu Wang, Jingqin Zhong, Kangjie Shen, Xinyi Lin, Zixu Gao, Wanlin Liu, Yinlam Li, Yu Xu, Ming Ren, Tu Hu, Chuanyuan Wei, Jianying Gu, Yong Chen
文章名称: An advanced comprehensive muti-cell-type-specific model for predicting anti-PD-1 therapeutic effect in melanoma
杂志: Theranostics
IF: 12.4
发表年份: 2024
发表日期: 2024 Mar 03
Abstract: [Abstract]
类型: 客户发文
平台: 10X Genomics
备注: 材料和方法提及Shbio,试剂盒名字
关键词: 抗PD-1，免疫检查点抑制剂，机器学习，恶性黑色素瘤，肿瘤微环境
分类: 疾病
研究内容: 机制
病种: 恶性黑色素瘤
采样物种分类: 人
通讯作者: 孙伟
单位: 复旦肿瘤医院
区域: 华东

IF 15.1 Advanced Science 2024

Radiotherapy-Induced Astrocyte Senescence Promotes anImmunosuppressive Microenvironment in Glioblastoma to Facilitate Tumor Regrowth

产品页

序号: 2436
分类: 单细胞
原文: The nuclei from the frozen brain tissues were isolated using Shbio Nuclei Isolation Kit (SHBIO; Shanghai, China).
应用方向: snRNA-seq
产品线: 单细胞/试剂
服务种类: 单细胞核转录组测序，细胞核分离试剂盒
使用服务种类: 单细胞核转录组测序、伯优®细胞核分离试剂盒
样本类型: 组织
样本详情: 大脑
作者名: Jianxiong Ji,* Kaikai Ding, Bo Cheng, Xin Zhang, Tao Luo, Bin Huang, Hao Yu, Yike Chen, Xiaohui Xu, Haopu Lin, Jiayin Zhou, Tingtin Wang, Mengmeng Jin, Aixia Liu, Danfang Yan, Fuyi Liu, Chun Wang, Jingsen Chen, Feng Yan, Lin Wang, Jianmin Zhang, Senxiang Yan,* Jian Wang,* Xingang Li,* and Gao Chen
文章名称: Radiotherapy-Induced Astrocyte Senescence Promotes anImmunosuppressive Microenvironment in Glioblastoma to Facilitate Tumor Regrowth
杂志: Advanced Science
IF: 15.1
发表年份: 2024
发表日期: 2024 Feb 11
Abstract: [Abstract]
类型: 客户发文
备注: 材料和方法提及Shbio
分类: 疾病
研究内容: 机制
病种: 胶质母细胞瘤
采样物种分类: 小鼠
通讯作者: Xingang Li
单位: 山东大学齐鲁医院
区域: 华北

IF 15.1 Advanced Science 2023

Single-Nucleus Transcriptome Profiling of Locally Advanced Cervical Squamous Cell Cancer Identifies Neural-Like Progenitor Program Associated with the Efficacy of Radiotherapy

产品页

序号: 2324
分类: 单细胞
原文: The nuclear fraction wasisolated usingaShbioCellNuclearIsolationKit(Shbio,52009-10,Shanghai, China
应用方向: snRNA-seq
产品线: 单细胞/试剂
服务种类: 单细胞核转录组测序/ 伯优细胞核分离试剂盒
样本类型: 组织
样本详情: 宫颈肿瘤
作者名: Lei Zhang, Jun Ma, Di Zhou, Junjun Zhou, Bin Hu, Xiumei Ma, Jianming Tang, Yongrui Bai,Haiyan Chen, and Ying Jing
文章名称: Single-Nucleus Transcriptome Profiling of Locally Advanced Cervical Squamous Cell Cancer Identifies Neural-Like Progenitor Program Associated with the Efficacy of Radiotherapy
杂志: Advanced Science
IF: 15.1
发表年份: 2023
发表日期: 2023 Jul 09
Abstract: [Abstract]
类型: 客户发文
平台: 10× Genomics
备注: 材料和方法提及Shanghai Biotechnology Corporation
分类: 疾病
研究内容: 机制
病种: 宫颈癌
采样物种分类: 人
通讯作者: 张蕾
单位: 仁济东院
区域: 华东

IF 4.6 JOURNAL OF DERMATOLOGICAL SCIENCE 2023

Updated skin transcriptomic atlas depicted by reciprocal contribution of single-nucleus RNA sequencing and single-cell RNA sequencing

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序号: 2312
分类: 单细胞
原文: The nuclei were separated with a Shbio Cell Nuclear Isolation Kit (Shbio, #52009–10, Shanghai, China)
应用方向: snRNA-seq
产品线: 单细胞/试剂
服务种类: 抽核试剂、单细胞核测序
使用服务种类: Shbio Cell Nuclear Isolation Kit
样本类型: 组织
样本详情: 皮肤
作者名: Ronghui Zhu, Xiaoyu Pan, Shangshang Wang, Zhuoqiong Qiu, Chaoying Gu, Xu Yao, Wei Li
文章名称: Updated skin transcriptomic atlas depicted by reciprocal contribution of single-nucleus RNA sequencing and single-cell RNA sequencing
杂志: JOURNAL OF DERMATOLOGICAL SCIENCE
IF: 4.6
发表年份: 2023
发表日期: 2023 Jun 20
Abstract: [Abstract]
类型: 客户发文
备注: 材料和方法提及shbio
关键词: 皮肤，单细胞RNA测序，单核RNA测序，角质形成细胞，成纤维细胞
分类: 基础
采样物种分类: 人
通讯作者: 李巍
单位: 复旦大学附属华山医院
区域: 华东

IF 13.437 MOLECULAR PSYCHIATRY 2022

Development of opioid-induced hyperalgesia depends on reactive astrocytes controlled by Wnt5a signaling

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序号: 2205
分类: 试剂
原文: The nucleus was isolated using a Nucleus Isolation Kit (catalogue no. 52009-10, SHBIO, China)
应用方向: snRNA-seq
产品线: 试剂
服务种类: 细胞核分离试剂盒
使用服务种类: Nucleus Isolation Kit
样本类型: 组织
样本详情: 脑
作者名: Liu, Xin, Bae, Chilman, Liu, Bolong, Zhang, Yong-Mei, Zhou, Xiangfu, Zhang, Donghang, Zhou, Cheng, DiBua, Adriana, Schutz, Livia, Kaczocha, Martin, Puopolo, Michelino, Yamaguchi, Terry P., Chung, Jin Mo, Tang, Shao-Jun
文章名称: Development of opioid-induced hyperalgesia depends on reactive astrocytes controlled by Wnt5a signaling
杂志: MOLECULAR PSYCHIATRY
IF: 13.437
发表年份: 2022
发表日期: 2022 Oct 06
刊/卷/页: 2022 Oct;:1-13
Abstract: [Abstract]
类型: 客户发文
平台: 10x Genomics
备注: 材料和方法提及Nucleus Isolation Kit (catalogue no. 52009-10, SHBIO, China)
分类: 疾病
研究内容: 机制
病种: 疼痛
采样物种分类: 人
通讯作者: Shao-Jun Tang
单位: 美国石溪大学疼痛与麻醉研究中心（SPARC）

IF 14.224 Journal Of Hazardous Materials 2022

Brain single-nucleus transcriptomics highlights that polystyrene nanoplastics potentially induce Parkinson’s Disease-like neurodegeneration by causing energy metabolism disorders in mice

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序号: 2075
分类: 单细胞
原文: Shbio Cell Nuclear Isolation Kit (Shbio, #52009–10, Shanghai, China)
应用方向: snRNA-seq
产品线: 单细胞/试剂
服务种类: 单细胞核转录组测序
使用服务种类: snRNA-seq/抽核试剂
样本类型: 组织
样本详情: 脑组织
作者名: Boxuan Liang, Yuji Huang, Yizhou Zhong, Zhiming Li, Rongyi Ye, Bo Wang,Bingli Zhang, Hao Meng, Xi Lin, Jiaxin Du, Manjiang Hu, Qinghong Wu, Haixia Sui, Xingfen Yang d, Zhenlie Huang
文章名称: Brain single-nucleus transcriptomics highlights that polystyrene nanoplastics potentially induce Parkinson’s Disease-like neurodegeneration by causing energy metabolism disorders in mice
杂志: Journal Of Hazardous Materials
IF: 14.224
发表年份: 2022
发表日期: 2022 Feb 11
Abstract: [Abstract]
类型: 客户发表
平台: 10X
备注: 致谢提及Shanghai Biotechnology Corporation
关键词: 纳米塑料，单核转录组学，帕金森病，神经变性，能量代谢紊乱
分类: 材料
病种: 神经退行性疾病
采样物种分类: 小鼠
通讯作者: 黄振烈
单位: 南方医科大学
区域: 华南

IF 6.4 Elife 2021

Single-nucleus transcriptomic atlas of spinal cord neuron in human

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序号: 2026
分类: 单细胞
原文: Nuclei were isolated using a Nucleus Isolation Kit (catalogue no. 52009-10, SHBIO, CHINA)
应用方向: snRNA-seq
产品线: 试剂/单细胞（核）
服务种类: SC-SEQ，单细胞抽核试剂
使用服务种类: 单细胞抽核试剂
样本类型: 人
样本详情: 组织
作者名: Donghang Zhang, Yiyong Wei, Jin Liu, Hongjun Chen, Jin Li, Tao Zhu, Cheng Zhou
文章名称: Single-nucleus transcriptomic atlas of spinal cord neuron in human
杂志: Elife
IF: 6.4
发表年份: 2021
发表日期: 2021 September 30
Abstract: [Abstract]
类型: 客户发表
平台: 10x
备注: 材料和方法提及 Nucleus Isolation Kit (catalogue no. 52009-10, SHBIO, CHINA)
关键词: 人，脊髓；单核RNA测序；神经元簇
分类: 神经
研究内容: 机制
采样物种分类: 人
通讯作者: Cheng Zhou，Jin Liu，Tao Zhu
单位: 华西医院
区域: 华南

产品问答

Q: 52009和52201都是针对组织样本通用款，两款抽核试剂盒流程及原理、起始量方面有什么区别？
A: 两款试剂盒基本操作流程均为：组织匀浆及细胞裂解、碎片去除、清洗、重悬细胞核。52009通过密度梯度法去除碎片及杂质，获得的细胞核纯度高；52201柱提法通过过滤柱及碎片去除液去除碎片及杂质，操作流程短，得率高。建议起始量为20-50mg，52201兼容10mg以下低起始量。

Q: 52009试剂盒单次测试需要多少组织？组织研磨用什么方法？
A：组织起始量建议20-50mg，研磨方法可根据组织类型选择电动匀浆仪研磨或研磨杵手动研磨。

Q: 小鼠肝抽核，后续做ATAC-seq。新鲜与冰冻组织后续是否有区别？是否有方法能保存新鲜组织？
A: 相对来说新鲜组织更好，冰冻组织液也可以做。单细胞测序组织保存液（伯优，21903）可在72h内保持组织细胞活性。

Q: 52009处理冰冻组织和新鲜组织都可以吗？
A: 新鲜和冰冻组织都可以的。

Q: 肿瘤样本，后续单细胞转录组，推荐用什么研磨匀浆方法？
A:推荐使用电动匀浆仪（Omni TH 手持/台式两用均质器）低转速研磨5-10s。

Q: 分离细胞核的组织可以冻融几次？
A: 分离的细胞核不建议冻融，建议半小时内进行后续实验。

Q: 某些样本匀浆无法完全匀浆，如何改善？
A: 可先用剪刀剪碎组织，再进行匀浆。大部分组织匀浆即可，过度匀浆也会影响细胞核形态及得率。

Q: Both 52009 and 52201 are general kits for tissue samples. What are the differences between them in workflow, principle and initial sample amount?
A: The basic workflow of both kits includes tissue homogenization & cell lysis, debris removal, washing and nucleus resuspension. Kit 52009 removes debris and impurities via density gradient method, delivering nuclei with high purity. Kit 52201 adopts column-based purification using filter columns and debris removal solution, featuring a shorter workflow and higher yield. The recommended initial sample amount is 20–50 mg. Kit 52201 is also compatible with low-input samples below 10 mg.

Q: How much tissue is required for a single test with Kit 52009? What methods are available for tissue grinding?
A: The recommended initial tissue amount is 20–50 mg. Either an electric homogenizer or a manual pestle can be used for grinding, depending on tissue type.

Q: For nucleus extraction from mouse liver samples followed by ATAC-seq, are there any differences between fresh and frozen tissues? Is there a solution to preserve fresh tissues?
A: Fresh tissues are preferred, while frozen tissues are also applicable. Single-cell Tissue Preservation Solution (Boyou, Cat. 21903) can maintain cellular activity for up to 72 hours.

Q: Can Kit 52009 be used for both frozen and fresh tissues?
A: Yes, it works for both fresh and frozen tissues.

Q: For tumor samples intended for subsequent single-cell RNA-seq, which grinding and homogenization method is recommended?
A: Use an electric homogenizer (Omni TH Handheld/Benchtop Homogenizer) at low speed for 5–10 seconds.

Q: How many freeze-thaw cycles are allowed for isolated nuclei?
A: Repeated freeze-thaw of isolated nuclei is not recommended. Subsequent experiments should be performed within half an hour.

Q: How to improve incomplete tissue homogenization for certain samples?
A: Cut the tissue into small pieces with scissors prior to homogenization. Moderate homogenization is sufficient; excessive homogenization will impair nucleus morphology and yield.

伯优^®细胞核分离试剂盒

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